Open AccessA hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development
Author(s) -
Zhaoqiang Wang,
Yichen Ding,
Sandro Satta,
M H Roustaei,
Peng Fei,
Tzung K. Hsiai
Publication year - 2021
Publication title -
plos computational biology/plos computational biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.628
H-Index - 182
eISSN - 1553-7358
pISSN - 1553-734X
DOI - 10.1371/journal.pcbi.1009175
Subject(s) - intracardiac injection , blood flow , cardiac cycle , velocimetry , light sheet fluorescence microscopy , cardiac imaging , particle image velocimetry , biomedical engineering , cardiac function curve , medicine , microscopy , optics , radiology , physics , pathology , turbulence , scanning confocal electron microscopy , thermodynamics , heart failure
Biomechanical forces intimately contribute to cardiac morphogenesis. However, volumetric imaging to investigate the cardiac mechanics with high temporal and spatial resolution remains an imaging challenge. We hereby integrated light-field microscopy (LFM) with light-sheet fluorescence microscopy (LSFM), coupled with a retrospective gating method, to simultaneously access myocardial contraction and intracardiac blood flow at 200 volumes per second. While LSFM allows for the reconstruction of the myocardial function, LFM enables instantaneous acquisition of the intracardiac blood cells traversing across the valves. We further adopted deformable image registration to quantify the ventricular wall displacement and particle tracking velocimetry to monitor intracardiac blood flow. The integration of LFM and LSFM enabled the time-dependent tracking of the individual blood cells and the differential rates of segmental wall displacement during a cardiac cycle. Taken together, we demonstrated a hybrid system, coupled with our image analysis pipeline, to simultaneously capture the myocardial wall motion with intracardiac blood flow during cardiac development.