Reprogramming efficiency and quality of induced Pluripotent Stem Cells (iPSCs) generated from muscle-derived fibroblasts of mdx mice at different ages | Zendy

Bo Wang | Zendy; Yuko MiyagoeSuzuki | Zendy; Erica Yada | Zendy; Naoki Ito | Zendy; Takashi Nishiyama | Zendy; Miho Nakamura | Zendy; Yusuke Ono | Zendy; Norio Motohashi | Zendy; Makoto Segawa | Zendy; S. Masuda | Zendy; Shin’ichi Takeda | Zendy

Open Access

Reprogramming efficiency and quality of induced Pluripotent Stem Cells (iPSCs) generated from muscle-derived fibroblasts of mdx mice at different ages

Author(s) -

Bo Wang,

Yuko MiyagoeSuzuki,

Erica Yada,

Naoki Ito,

Takashi Nishiyama,

Miho Nakamura,

Yusuke Ono,

Norio Motohashi,

Makoto Segawa,

S. Masuda,

Shin’ichi Takeda

Publication year - 2011

Publication title -

plos currents

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.282

H-Index - 49

ISSN - 2157-3999

DOI - 10.1371/currents.rrn1274

Subject(s) - induced pluripotent stem cell , reprogramming , homeobox protein nanog , duchenne muscular dystrophy , microbiology and biotechnology , mdx mouse , muscular dystrophy , stem cell , biology , embryonic stem cell , medicine , cell , dystrophin , genetics , gene

Induced pluripotent stem cells (iPSCs) hold promise as a potential treatment for Duchenne muscular dystrophy (DMD). To determine the impact of the donor’s age on reprogramming, we generated iPSCs from muscle-derived fibroblasts (MuFs) of mdx mice aged 6 weeks, 6 months, and 14 months. MuFs from 14-month-old mdx mice showed lower proliferative activity and lower reprogramming efficiency, compared with those from younger mdx mice. Furthermore, iPSCs derived from 14-month-old mdx mice (14m-MuF-iPSCs) gradually lost Nanog expression, and regressed in conventional ES medium during passages. Interestingly, inhibition of TGF-β signaling and BMP signaling stabilized Nanog expression and promoted self-renewal of 14m-MuF-iPSCs. Finally, rescued mdx-derived iPSCs efficiently differentiated into the skeletal muscle lineage.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research