Open AccessRemote focusing in a temporal focusing microscope
Author(s) -
Michael Durst,
Samuel Yurak,
Joe Moscatelli,
Isabel Linhares,
Ruben R. Vargas
Publication year - 2021
Publication title -
osa continuum
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 8
ISSN - 2578-7519
DOI - 10.1364/osac.443116
Subject(s) - microscope , cardinal point , optics , focus (optics) , lens (geology) , axial symmetry , plane (geometry) , image plane , focal length , dispersion (optics) , excitation , microscopy , physics , computer science , materials science , artificial intelligence , image (mathematics) , mathematics , geometry , quantum mechanics
In a temporal focusing microscope, dispersion can remotely shift the temporal focal plane axially, but only a single depth can be in focus at a time on a fixed camera. In this paper, we demonstrate remote focusing in a temporal focusing microscope. Dispersion tuning with an electrically tunable lens (ETL) in a 4 f pulse shaper scans the excitation plane axially, and another ETL in the detection path keeps the shifted excitation plane in focus on the camera. Image stacks formed using two ETLs versus a traditional stage scan are equivalent.