Airy-beam tomographic microscopy | Zendy

Jian Wang | Zendy; Xuanwen Hua | Zendy; Changliang Guo | Zendy; Wenhao Liu | Zendy; Shu Jia | Zendy

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Airy-beam tomographic microscopy

Author(s) -

Jian Wang,

Xuanwen Hua,

Changliang Guo,

Wenhao Liu,

Shu Jia

Publication year - 2020

Publication title -

optica

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 5.074

H-Index - 107

ISSN - 2334-2536

DOI - 10.1364/optica.389894

Subject(s) - microscopy , optics , airy beam , tomographic reconstruction , diffraction , cardinal point , microscope , resolution (logic) , light sheet fluorescence microscopy , optical microscope , beam (structure) , tomography , physics , materials science , computer science , scanning confocal electron microscopy , artificial intelligence , scanning electron microscope

We introduce Airy-beam tomographic microscopy (ATM) for high-resolution, volumetric, inertia-free imaging of biological specimens. The work exploits the highly adjustable Airy trajectories in the three-dimensional (3D) space, transforming the conventional telecentric wide-field imaging scheme that requires sample or focal-plane scanning to acquire 3D information. The results present a consistent near-diffraction-limited 3D resolution across a tenfold extended imaging depth compared to wide-field microscopy. We anticipate the strategy to not only offer a promising paradigm for 3D optical microscopy, but also be translated to other non-optical waveforms.

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