Open AccessAiry-beam tomographic microscopy
Author(s) -
Jian Wang,
Xuanwen Hua,
Changliang Guo,
Wenhao Liu,
Shu Jia
Publication year - 2020
Publication title -
optica
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 5.074
H-Index - 107
ISSN - 2334-2536
DOI - 10.1364/optica.389894
Subject(s) - microscopy , optics , airy beam , tomographic reconstruction , diffraction , cardinal point , microscope , resolution (logic) , optical microscope , light sheet fluorescence microscopy , beam (structure) , tomography , materials science , physics , scanning confocal electron microscopy , computer science , artificial intelligence , scanning electron microscope
We introduce Airy-beam tomographic microscopy (ATM) for high-resolution, volumetric, inertia-free imaging of biological specimens. The work exploits the highly adjustable Airy trajectories in the three-dimensional (3D) space, transforming the conventional telecentric wide-field imaging scheme that requires sample or focal-plane scanning to acquire 3D information. The results present a consistent near-diffraction-limited 3D resolution across a tenfold extended imaging depth compared to wide-field microscopy. We anticipate the strategy to not only offer a promising paradigm for 3D optical microscopy, but also be translated to other non-optical waveforms.