Open AccessAll-solid-state lock-in imaging for wide-field fluorescence lifetime sensing
Author(s) -
Alessandro Esposito,
Thierry Oggier,
Hans C. Gerritsen,
Felix Lustenberger,
Fred S. Wouters
Publication year - 2005
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/opex.13.009812
Subject(s) - fluorescence lifetime imaging microscopy , optics , detector , materials science , cmos sensor , throughput , image sensor , diode , microscopy , fluorescence , lock (firearm) , biological imaging , optoelectronics , computer science , physics , telecommunications , wireless , mechanical engineering , engineering
Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique that is increasingly being used in the life sciences during the past decades. However, a broader application of FLIM requires more cost-effective and user-friendly solutions. We demonstrate the use of a simple CCD/CMOS lock-in imager for fluorescence lifetime detection. The SwissRanger SR-2 time-of-flight detector, originally developed for 3D vision, embeds all the functionalities required for FLIM in a compact system. The further development of this technology and its combination with light-emitting-and laser diodes could drive a wider spreading of thuse of FLIM including high-throughput applications.