Open AccessSelective detection of antibodies in microstructured polymer optical fibers
Author(s) -
Jørgen Birger Jensen,
Poul E. Høiby,
Grigoriy Emiliyanov,
Ole Bang,
Lars H. Pedersen,
Anders Bjarklev
Publication year - 2005
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/opex.13.005883
Subject(s) - materials science , fluorophore , optical fiber , biosensor , streptavidin , optics , polymer , photonic crystal fiber , biomolecule , fiber optic sensor , microstructured optical fiber , fiber , fluorescence , optoelectronics , nanotechnology , plastic optical fiber , chemistry , composite material , biochemistry , physics , biotin
We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fiber (mPOF). The fiber core is defined by a ring of 6 air holes and a simple procedure was applied to selectively capture either alpha-streptavidin or alpha-CRP antibodies inside these air holes. A sensitive and easy-to-use fluorescence method was used for the optical detection. Our results show that mPOF based biosensors can provide reliable and selective antibody detection in ultra small sample volumes.