Open AccessVibrationally resonant imaging of a single living cell by supercontinuum-based multiplex coherent anti-Stokes Raman scattering microspectroscopy
Author(s) -
Hideaki Kano,
Hiro O. Hamaguchi
Publication year - 2005
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/opex.13.001322
Subject(s) - supercontinuum , raman scattering , optics , raman spectroscopy , hyperspectral imaging , coherent anti stokes raman spectroscopy , spectral imaging , materials science , raman microspectroscopy , multiplex , scattering , fission , physics , optical fiber , photonic crystal fiber , biology , bioinformatics , remote sensing , quantum mechanics , neutron , geology
Supercontinuum-based multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy has been applied to vibrational imaging of a living fission yeast cell. We have successfully extracted only a vibrationally resonant CARS image from a characteristic spectral profile in the C-H stretching vibrational region. Using our simple but sensitive analysis, the vibrational contrast is significantly improved in comparison with a CARS imaging at a fixed Raman shift. The CARS image of a living yeast cell indicates several areas at which the signal is remarkably strong. They are considered to arise from mitochondria.