Open AccessUltrahigh resolution optical biopsy with endoscopic optical coherence tomography
Author(s) -
Paul R. Herz,
Yu Chen,
Aaron D. Aguirre,
James G. Fujimoto,
Hiroshi Mashimo,
Joseph M. Schmitt,
Amanda Koski,
J. Goodnow,
Chris Petersen
Publication year - 2004
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/opex.12.003532
Subject(s) - optical coherence tomography , preclinical imaging , optical tomography , optics , materials science , biomedical engineering , tomography , medical imaging , in vivo , radiology , medicine , physics , biology , microbiology and biotechnology
Optical coherence tomography (OCT) is an emerging medical imaging technology that can generate high resolution, cross-sectional images of tissue in situ and in real time. Although endoscopic OCT has been used successfully to identify certain pathologies in the gastrointestinal tract, the resolution of current endoscopic OCT systems has been limited to 10-15 microm for in vivo studies. In this study, in vivo imaging of the rabbit gastrointestinal tract is demonstrated at a three-fold higher resolution (< 5 microm), using a broadband Cr(4+):Forsterite laser as the optical light source. Images acquired from the esophagus, trachea, and colon reveal high resolution details of tissue architecture. Definitive correlation of architectural features in OCT images and histological sections is shown. The ability of ultrahigh resolution endoscopic OCT to image tissue morphology at an unprecedented resolution in vivo advances the development of OCT as a potential imaging tool for the early detection of neoplastic changes in biological tissue.