Open AccessConfocal laser scanning fluorescence microscopy with a visible continuum source
Author(s) -
Gail McConnell
Publication year - 2004
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/opex.12.002844
Subject(s) - materials science , optics , femtosecond , laser , light sheet fluorescence microscopy , photonic crystal fiber , confocal , microscopy , confocal microscopy , photonic crystal , microscope , sapphire , fluorescence , laser scanning , fluorescence microscope , fiber laser , optoelectronics , wavelength , scanning confocal electron microscopy , physics
Confocal laser scanning fluorescence microscopy is demonstrated using a photonic crystal fiber-based excitation source. A 38 cm-long section of photonic crystal fiber is pumped with femtosecond pulses from a Ti:sapphire laser, and the resultant visible continuum is selectively filtered to provide the peak excitation wavelengths required for a range of fluorescently labeled biological tissue.