Open AccessDetection of biological tissue in gels using pulsed digital holography
Author(s) -
María del Socorro Hernández-Montes,
Carlos PérezLópez,
Fernando Mendoza Santoyo,
Luis Manuel Muñoz Guevara
Publication year - 2004
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/opex.12.000853
Subject(s) - materials science , transient (computer programming) , shaker , optics , holography , phase (matter) , homogeneous , digital holography , displacement (psychology) , ultrasonic sensor , acoustics , physics , vibration , psychology , quantum mechanics , computer science , psychotherapist , thermodynamics , operating system
An out of plane optical sensitive configuration for pulsed digital holography was used to detect biological tissue inside solid organic materials like gels. A loud speaker and a shaker were employed to produce a mechanical wave that propagates through the gel in such a way that it generates vibrational resonant modes and transient events on the gel surface. Gel surface micro displacements were observed between the firing of two laser pulses, both for a steady resonant mode and for different times during the transient event. The biological tissue sample inserted approximately 2 cm inside the gel diffracts the original mechanical wave and changes the resonant mode pattern or the transient wave on the gel surface. This fact is used to quantitatively measure the gel surface micro displacement. Comparison of phase unwrapped patterns, with and without tissue inside the gel, allows the rapid identification of the existence of tissue inside the gel. The results for the resonant and transient conditions show that the method may be reliably used to study, compare and distinguish data from inside homogeneous and in-homogeneous solid organic materials.