Open AccessTotal internal reflection enabled wide-field coherent anti-Stokes Raman scattering microscopy
Author(s) -
Benjamin Doughty,
Uvinduni I. Premadasa,
John F. Cahill,
Amber B. Webb,
Jennifer L. MorrellFalvey,
Muneeba Khalid,
Scott T. Retterer,
YingZhong Ma
Publication year - 2020
Publication title -
optics letters/optics index
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.45.003087
Subject(s) - optics , raman scattering , total internal reflection , microscopy , materials science , scattering , reflection (computer programming) , raman spectroscopy , physics , computer science , programming language
Wide-field coherent anti-Stokes Raman scattering (CARS) microscopy offers an attractive means for the rapid and simultaneous acquisition of vibrationally resolved images across a large field of view. A major challenge in the implementation lies in how to achieve sufficiently strong excitation fields necessary to drive the third-order optical responses over the large focal region. Here, we report a new wide-field CARS microscope enabled by a total internal reflection excitation scheme using a femtosecond Ti:Sapphire oscillator to generate pump and broadband near-infrared Stokes pulses. The spectrally broad Stokes pulse, in combination with its inherent chirp, offers not only access to a wide range of Raman modes spanning ∼1000 to ∼3500 c m -1 but also a straightforward means to select vibrational transitions within this range by simply varying the time delay between the pulses. The unique capabilities of this wide-field CARS microscope were validated by acquiring high-quality CARS images from the model and complex biological samples on conventional microscope coverslips.