Open AccessHigh-sensitivity imaging and quantification of intratumoral distributions of gold nanoparticles using a benchtop x-ray fluorescence imaging system
Author(s) -
Nivedh Manohar,
Francisco J. Reynoso,
Sandun Jayarathna,
Hem Moktan,
Md. Foiez Ahmed,
Parmeswaran Diagaradjane,
Sunil Krishnan,
Sang Hyun Cho
Publication year - 2019
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.44.005314
Subject(s) - ex vivo , colloidal gold , fluorescence lifetime imaging microscopy , preclinical imaging , materials science , fluorescence , molecular imaging , in vivo , medical imaging , gold standard (test) , optics , nanoparticle , biomedical engineering , nanotechnology , medicine , radiology , physics , microbiology and biotechnology , biology
A high-sensitivity benchtop x-ray fluorescence (XRF) imaging system, based on a high-power x-ray source and silicon drift detector, has been developed. This system allows gold L-shell XRF-based quantitative imaging of gold nanoparticles (GNPs) at concentrations as low as 0.007 mg/cm 3 (7 ppm) in biological tissues/water. Its capability for biomedical applications was demonstrated by imaging the GNP distribution within a small (∼12×11×2 mm 3 ) ex vivo sample (extracted from a murine tumor after intravenous GNP administration). The results suggest direct translatability for routine preclinical ex vivo imaging tasks involving GNPs, as well as the possibility for in vivo imaging of small/superficial animal tumors.