Open AccessElectrically tunable lens integrated with optical coherence tomography angiography for cerebral blood flow imaging in deep cortical layers in mice
Author(s) -
Yuandong Li,
Ping Tang,
Shaozhen Song,
Adiya Rakymzhan,
Ke Wang
Publication year - 2019
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.44.005037
Subject(s) - optical coherence tomography , optics , lens (geology) , blood flow , microangiography , materials science , optical coherence tomography angiography , depth of field , biomedical engineering , physics , medicine
We report the use of an electrically tunable lens (ETL) in a 1.3 μm spectral-domain optical coherence tomography (SD-OCT) system to overcome the depth of focus (DOF) limitation in conventional OCT systems for OCT angiography (OCTA) in a mouse cerebral cortex. The ETL provides fast and dynamic control of the axial focus of the probe beam along the entire range of the mouse cortex, upon which we performed cerebral blood flow imaging of all cortical layers by stitching the OCTA images automatically captured at six focal depths. Capillary vasculature and axial blood flow velocity were revealed in distinctive cortical layers and, for the first time, to the best of our knowledge, in white matter. The results have shown the system capability to conveniently investigate the hemodynamics in deep cortical layers in the mouse brain. More importantly, the compact integration of an ETL will benefit the future design of handheld or intra-cavity OCT probes for a wide range of applications in research and clinical fields.