Open AccessFiber-based platform for synchronous imaging of endogenous and exogenous fluorescence of biological tissue
Author(s) -
Alba Alfonso-García,
Cai Li,
Julien Bec,
Diego R. Yankelevich,
Laura Marcu,
Benjamin E. Sherlock
Publication year - 2019
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.44.003350
Subject(s) - autofluorescence , fluorescence , fluorescence lifetime imaging microscopy , optics , materials science , optical fiber , chopper , multi mode optical fiber , excitation , physics , voltage , quantum mechanics
Endogenous and exogenous fluorescence emission from biological samples encodes complementary information. Here we report, to the best of our knowledge, the first results from an optical imaging platform with interleaved excitation and detection of exogenous and endogenous fluorescence from tissue samples using a single flexible multimode fiber that delivers the excitation beam and collects the emitted light. A custom-built reflective optical chopper wheel with synchronized rotation temporally multiplexes an autofluorescence lifetime imaging apparatus with an intensity-based fluorescence module tailored to imaging green fluorescent protein. We demonstrate the functionality of such platform imaging dyes of varying fluorescence signatures and resolving cellularized areas on bio-engineered tissue constructs.