Open AccessMultiscale, multispectral fluorescence lifetime imaging using a double-clad fiber
Author(s) -
Benjamin E. Sherlock,
Cai Li,
Xiaoliang Zhou,
Alba Alfonso-García,
Julien Bec,
Diego R. Yankelevich,
Laura Marcu
Publication year - 2019
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.44.002302
Subject(s) - multispectral image , optics , autofluorescence , point spread function , materials science , fluorescence lifetime imaging microscopy , fluorescence , image resolution , fiber , spectral imaging , optical fiber , resolution (logic) , biomedical engineering , computer science , remote sensing , physics , geology , artificial intelligence , composite material , medicine
Fiber-based imaging of tissue autofluorescence using ultraviolet (UV) excitation is a highly flexible tool used to probe structure and composition. In this Letter, we report, to the best of our knowledge, the first results from a single-fiber imaging system employing a custom double-clad fiber to acquire multispectral fluorescence lifetime images at two distinct spatial resolutions. We characterize the lateral point spread function and fluorescent background of the system and show how enhanced resolution can identify features such as trabeculae in ex vivo murine bone samples.