Open AccessDiffusion-based single-shot diffraction tomography
Author(s) -
Ryoichi Horisaki,
Kazuki Fujii,
Jun Tanida
Publication year - 2019
Publication title -
optics letters/optics index
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.44.001964
Subject(s) - optics , holography , diffuser (optics) , single shot , phase retrieval , microscopy , microscope , digital holographic microscopy , spatial light modulator , diffraction , phase (matter) , computer science , physics , fourier transform , light source , quantum mechanics
Holographic microscopy is a powerful technique for noninvasive label-free biomedical imaging. Most holographic methods utilize reference light and/or multiple measurements to observe both the amplitude and phase of a light wave passing through a specimen. However, such fundamental requirements degrade the spatial resolution due to the use of a reference carrier, cause difficulties for real-time imaging of dynamic biological events, and make the optical setups bulky. Here, we realized reference-free, single-shot holographic tomography by just inserting a diffuser into the optical path in a conventional microscope setup to generate randomly structured illumination. A three-dimensional complex amplitude field was reconstructed from a single scattered intensity image by means of sparsity-constrained multislice phase retrieval.