Open AccessMultimodal high-resolution embryonic imaging with light sheet fluorescence microscopy and optical coherence tomography
Author(s) -
Behzad Khajavi,
Ruijiao Sun,
Harshdeep Singh Chawla,
Henry Le,
Manmohan Singh,
Alexander Schill,
Mary E. Dickinson,
David Mayerich,
Kirill V. Larin
Publication year - 2021
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.430202
Subject(s) - optical coherence tomography , light sheet fluorescence microscopy , optics , microscopy , materials science , fluorescence microscope , fluorescence lifetime imaging microscopy , fluorescence , resolution (logic) , coherence (philosophical gambling strategy) , physics , quantum mechanics , artificial intelligence , computer science
A high-resolution imaging system combining optical coherence tomography (OCT) and light sheet fluorescence microscopy (LSFM) was developed. LSFM confined the excitation to only the focal plane, removing the out of plane fluorescence. This enabled imaging a murine embryo with higher speed and specificity than traditional fluorescence microscopy. OCT gives information about the structure of the embryo from the same plane illuminated by LSFM. The co-planar OCT and LSFM instrument was capable of performing co-registered functional and structural imaging of mouse embryos simultaneously.