Open AccessPatterned-illumination second harmonic generation microscopy of collagen fibrils in rat scleras
Author(s) -
Ben E. Urban,
Biqin Dong,
Xian Zhang,
HuiLi Yang,
Hao F. Zhang
Publication year - 2018
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.43.005190
Subject(s) - microscopy , second harmonic generation , optics , materials science , optical microscope , resolution (logic) , collagen fibril , second harmonic imaging microscopy , super resolution microscopy , light sheet fluorescence microscopy , scanning confocal electron microscopy , scanning electron microscope , biophysics , laser , physics , artificial intelligence , computer science , biology
We developed a patterned-illumination second harmonic generation (PI-SHG) microscopy, which combines the principle of structured illumination reconstruction with SHG microscopy for label-free super-resolution imaging. We confirmed that PI-SHG microscopy can achieve 1.59-time resolution improvement compared to conventional SHG microscopy by imaging nanowire samples. We further demonstrated three-dimensional PI-SHG microscopy in imaging ex vivo collagen fibrils in rat scleras.