Open AccessAngle-insensitive amorphous silicon optical filter for fluorescence contact imaging
Author(s) -
Efthymios P. Papageorgiou,
Hui Zhang,
Bernhard E. Boser,
Catherine Park,
Mekhail Anwar
Publication year - 2018
Publication title -
optics letters/optics index
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.43.000354
Subject(s) - optics , materials science , fluorescence , amorphous silicon , filter (signal processing) , absorption (acoustics) , wavelength , silicon , fluorescence lifetime imaging microscopy , excitation wavelength , optical filter , light sheet fluorescence microscopy , optoelectronics , crystalline silicon , fluorescence microscope , physics , computer science , computer vision
We introduce a novel amorphous silicon absorption filter that has high rejection for all angles of incident light for wavelengths below approximately 700 nm. This filter is used for microscopic cancer tissue detection in a small intraoperative contact fluorescence imaging system that requires excitation light at oblique angles. Our 15 μm thick filter presents over five orders of magnitude rejection at 633 nm, making it compatible with several clinically tested fluorophores, including IR700DX. We have demonstrated imaging of fluorescently labeled human epidermal growth factor receptor 2+ breast cancer tissue using the filter, and we can reliably detect microscopic clusters of breast cancer cells with only a 75 ms integration time.