Open AccessWide-field imaging combined with confocal microscopy using a miniature f/5 camera integrated within a high NA objective lens
Author(s) -
David L. Dickensheets,
Seth Kreitinger,
Gary Peterson,
Michael Heger,
Milind Rajadhyaksha
Publication year - 2017
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.42.001241
Subject(s) - optics , confocal , confocal microscopy , lens (geology) , microscopy , optical sectioning , materials science , microscope , bright field microscopy , camera lens , resolution (logic) , field of view , physics , computer science , artificial intelligence
Wide-field (WF) imaging paired with reflectance confocal microscopy can noninvasively detect skin cancer with high accuracy. However, two separate devices are required to perform each imaging procedure. We describe a new concept that integrates the two into one device: a miniature WF color camera within the objective lens used for confocal microscopy, providing simultaneous sub-surface cellular imaging and WF surface morphologic imaging. The camera, inserted between a hyperhemisphere front lens and a back lens group of the objective, commands a field of view of 4.0 mm, with a resolution better than 30 μm, while confocal optical sectioning is preserved at sharper than 2.5 μm.