Photoswitchable stimulated Raman scattering spectroscopy and microscopy | Zendy

Jingwen Shou | Zendy; Yasuyuki Ozeki | Zendy

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Photoswitchable stimulated Raman scattering spectroscopy and microscopy

Author(s) -

Jingwen Shou,

Yasuyuki Ozeki

Publication year - 2021

Publication title -

optics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.524

H-Index - 272

eISSN - 1071-2763

pISSN - 0146-9592

DOI - 10.1364/ol.418240

Subject(s) - raman scattering , raman spectroscopy , optics , microscopy , materials science , narrowband , spectroscopy , multiplexing , scattering , coherent anti stokes raman spectroscopy , optoelectronics , physics , computer science , telecommunications , quantum mechanics

Photoswitchable fluorescence is a powerful technique to realize super-resolution imaging, highlighting, and optical storage, while its multiplexing capability is limited. Raman scattering is attracting attention because it generates narrowband vibrational signatures, which are potentially useful for highly multiplexed detection of different constituents. Here, we demonstrate photoswitchable stimulated Raman scattering (SRS) spectroscopy and microscopy where narrowband vibrational signatures are switched with full reversibility at high speed. The demonstration of live-cell photoswitchable SRS imaging shows good sensitivity and compatibility with biological living systems.

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