Open AccessPolarization sensitive optical coherence microscopy for brain imaging
Author(s) -
Hui Wang,
Taner Akkin,
Caroline Magnain,
Ruopeng Wang,
Jay Dubb,
William J. Kostis,
Mohammad A. Yaseen,
Avilash Cramer,
Sava Sakadžić,
David A. Boas
Publication year - 2016
Publication title -
optics letters/optics index
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.41.002213
Subject(s) - optics , optical coherence tomography , birefringence , microscopy , materials science , polarization (electrochemistry) , coherence (philosophical gambling strategy) , transverse plane , microscope , physics , chemistry , medicine , anatomy , quantum mechanics
Optical coherence tomography (OCT) and optical coherence microscopy (OCM) have demonstrated the ability to investigate cyto- and myelo-architecture in the brain. Polarization-sensitive OCT provides sensitivity to additional contrast mechanisms, specifically the birefringence of myelination and, therefore, is advantageous for investigating white matter fiber tracts. In this Letter, we developed a polarization-sensitive optical coherence microscope (PS-OCM) with a 3.5 μm axial and 1.3 μm transverse resolution to investigate fiber organization and orientation at a finer scale than previously demonstrated with PS-OCT. In a reconstructed mouse brain section, we showed that at the focal depths of 20-70 μm, the PS-OCM reliably identifies the neuronal fibers and quantifies the in-plane orientation.