Open AccessVolumetric two-photon fluorescence imaging of live neurons using a multimode optical fiber
Author(s) -
Raphaël Turcotte,
Carla C. Schmidt,
Martin J. Booth,
Nigel J. Emptage
Publication year - 2020
Publication title -
optics letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.409464
Subject(s) - optics , multi mode optical fiber , femtosecond , wavefront , preclinical imaging , materials science , two photon excitation microscopy , fluorescence lifetime imaging microscopy , chromatic aberration , optical fiber , laser , image resolution , fluorescence , physics , chromatic scale , in vivo , microbiology and biotechnology , biology
Multimode optical fibers (MMFs), combined with wavefront control methods, have achieved minimally invasive in vivo imaging of neurons in deep-brain regions with diffraction-limited spatial resolution. Here, we report a method for volumetric two-photon fluorescence imaging with a MMF-based system requiring a single transmission matrix measurement. Central to this method is the use of a laser source able to generate both continuous wave light and femtosecond pulses. The chromatic dispersion of pulses generated an axially elongated excitation focus, which we used to demonstrate volumetric imaging of neurons and their dendrites in live rat brain slices through a 60 µm core MMF.
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