Open AccessHigh-speed fiber-optic scanning nonlinear endomicroscopy for imaging neuron dynamics in vivo
Author(s) -
Hyeon-Cheol Park,
Honghua Guan,
Ang Li,
Yishi Yue,
Mingjun Li,
Hui Lü,
Xingde Li
Publication year - 2020
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.396023
Subject(s) - endomicroscopy , miniaturization , optics , preclinical imaging , confocal , materials science , fluorescence lifetime imaging microscopy , temporal resolution , optical fiber , two photon excitation microscopy , biomedical engineering , in vivo , physics , nanotechnology , fluorescence , medicine , biology , microbiology and biotechnology
Fiber-optic-based two-photon fluorescence endomicroscopy is emerging as an enabling technology for in vivo histological imaging of internal organs and functional neuronal imaging on freely-behaving animals. However, high-speed imaging remains challenging due to the expense of miniaturization and lack of suited fast beam scanners. For many applications, a higher imaging speed is highly desired, especially for monitoring functional dynamics such as transient dendritic responses in neuroscience. This Letter reports the development of a fast fiber-optic scanning endo-microscope with an imaging speed higher than 26 frames/s. In vivo neural dynamics imaging with the high-speed endomicroscope was performed on a freely-behaving mouse over the primary motor cortex that expressed GCaMP6m. The results demonstrate its capability of real-time monitoring of transient neuronal dynamics with very fine temporal resolution.