Open AccessPrecise 3D particle localization over large axial ranges using secondary astigmatism
Author(s) -
Yongzhuang Zhou,
Guillem Carles
Publication year - 2020
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.388695
Subject(s) - optics , astigmatism , microscopy , diffraction , aperture (computer memory) , phase (matter) , optical tweezers , numerical aperture , flexibility (engineering) , phase retrieval , physics , computer science , mathematics , fourier transform , wavelength , statistics , quantum mechanics , acoustics
We propose an analytical pupil phase function employing cropped secondary astigmatism for extended-depth nanoscale 3D-localization microscopy. The function provides high localization precision in all three dimensions, which can be maintained over extended axial ranges, customizable up to two orders of magnitude relative to the conventional, diffraction-limited imaging. This enables, for example, capturing nanoscale dynamics within a whole cell. The flexibility and simplicity in the implementation of the proposed phase function make its adoption in localization-based microscopy attractive. We demonstrate and validate its application to real-time imaging of 3D fluid flow over a depth of 40 µm with a numerical aperture of 0.8.