Open AccessSelf validating Mueller matrix Micro–Mesoscope (SAMMM) for the characterization of biological media
Author(s) -
Ilyas Saytashev,
S. Saha,
Joseph Chue-Sang,
Pedro F. Lopez,
Megan Laughrey,
Jessica C. RamellaRoman
Publication year - 2020
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.387747
Subject(s) - mueller calculus , polarimetry , optics , reflectivity , polarization (electrochemistry) , materials science , characterization (materials science) , confocal , microscopy , scattering , remote sensing , physics , chemistry , geology
Reflectance Mueller matrix (MM) polarimetry is being used to characterize biological media in multiple clinical applications. The origin of the reflectance polarimetric data is often unclear due to the impact of multiple scattering and tissue heterogeneity. We have developed a new, to the best of our knowledge, multimodal imaging technique combining MM reflectance, MM digital confocal imaging, and co-registered nonlinear microscopy techniques. The instrument unveils the origin of reflectance polarimetric signature in terms of confocal reflectance data. The reconstructed reflected MM demonstrates the capability of our method to provide depth-resolved 3D polarization response from complex biological media in terms of depolarization, retardance, and orientation parameters.