Open AccessIndocyanine green provides absorption and spectral contrast for optical coherence tomography at 840 nm in vivo
Author(s) -
Conrad W. Merkle,
Marco Augustin,
Danielle J. Harper,
Bernhard Baumann
Publication year - 2020
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.380051
Subject(s) - optical coherence tomography , contrast (vision) , optics , indocyanine green , materials science , fluorescence , coherence (philosophical gambling strategy) , absorption (acoustics) , preclinical imaging , in vivo , biomedical engineering , medicine , physics , microbiology and biotechnology , quantum mechanics , biology
In recent years, there has been growing interest in the application of exogenous contrast agents to supplement the traditional strengths of optical coherence tomography (OCT) and provide additional biological information. In this Letter, we present how indocyanine green, a common fluorescent contrast agent approved by the United States Food and Drug Administration, can provide absorption and spectral contrast for OCT imaging in the mouse eye in vivo. We further demonstrate high stability of spectral contrast measurements for the long-term monitoring of contrast agents in spite of fluctuations in intensity.