Open AccessHarmonically decoupled gradient light interference microscopy (HD-GLIM)
Author(s) -
Yi Wang,
Mikhail E. Kandel,
Michael J. Fanous,
Chenfei Hu,
Hsuan Yu Chen,
Xiaoxu Liu,
Gabriel Popescu
Publication year - 2020
Publication title -
optics letters/optics index
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.379732
Subject(s) - differential interference contrast microscopy , optics , interference microscopy , spatial light modulator , microscopy , interference (communication) , optical microscope , phase (matter) , materials science , differential phase , adaptive optics , phase contrast microscopy , physics , computer science , telecommunications , scanning electron microscope , channel (broadcasting) , quantum mechanics
Differential phase sensitive methods, such as Nomarski microscopy, play an important role in quantitative phase imaging due to their compatibility with partially coherent illumination and excellent optical sectioning ability. In this Letter, we propose a new system, to the best of our knowledge, to retrieve differential phase information from transparent samples. It is based on a 4f optical system with an amplitude-type spatial light modulator (SLM), which removes the need for traditional differential interference contrast (DIC) optics and specialized phase-only SLMs. We demonstrate the principle of harmonically decoupled gradient light interference microscopy using standard samples, as well as static and dynamic biospecimens.