Confocal microscopy with a high numerical aperture parabolic mirror | Zendy

Andreas Drechsler | Zendy; Max Lieb | Zendy; Christian Debus | Zendy; Alfred J. Meixner | Zendy; G. Tarrach | Zendy

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Confocal microscopy with a high numerical aperture parabolic mirror

Author(s) -

Andreas Drechsler,

Max Lieb,

Christian Debus,

Alfred J. Meixner,

G. Tarrach

Publication year - 2001

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.9.000637

Subject(s) - optics , confocal , parabolic reflector , numerical aperture , microscope , materials science , microscopy , resolution (logic) , confocal microscopy , light sheet fluorescence microscopy , image resolution , 4pi microscope , fluorescence , diffraction , scanning confocal electron microscopy , fluorescence microscope , physics , wavelength , multiphoton fluorescence microscope , artificial intelligence , computer science

A novel high-resolution stage scanning confocal microscope for fluorescence microscopy and spatially resolved spectroscopy with a high numerical aperture (NA 1) parabolic mirror objective is investigated. A spatial resolution close to the diffraction limit is achieved. As microscopic fluorescent test objects, dye-loaded zeolite microcrystals (diameter approx. 0.4 microm) and single fluorescent molecules were used. Confocal fluorescence images show a spatial resolution of .x = 0.8 . both at room temperature and at 1.8 K. Imaging of a quasi-point light source and focusing by the parabolic mirror were investigated experimentally and theoretically. Deviations between the theoretical results for a perfect parabolic mirror and the experimental results can be attributed to small deviations of the mirror profile from an ideal parabola.

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