Open AccessWidefield multiphoton and temporally decorrelated multifocal multiphoton microscopy
Author(s) -
D. N. Fittinghoff,
Paul W. Wiseman,
Jeff Squier
Publication year - 2000
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.7.000273
Subject(s) - optics , microscope , multiphoton fluorescence microscope , microscopy , materials science , ultrashort pulse , laser , fluorescence microscope , physics , fluorescence
We demonstrate a widefield multiphoton microscope and a temporally decorrelated, multifocal, multiphoton microscope that is based on a high-efficiency array of cascaded beamsplitters. Because these microscopes use ultrashort pulse excitation over large areas of the sample, they allow efficient use of the high-average power available from modern ultrashort pulse lasers.