Open AccessTowards stimulated Raman scattering spectro-microscopy across the entire Raman active region using a multiple-plate continuum
Author(s) -
GuanJie Huang,
Pang-Hsin Lai,
Mingwei Shen,
JiaXuan Su,
Jhan-Yu Guo,
Kuo-Chuan Chao,
Peng Lin,
JiXin Cheng,
LiAn Chu,
AnnShyn Chiang,
Bo-Han Chen,
ChihHsuan Lu,
ShiWei Chu,
ShangDa Yang
Publication year - 2022
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.469050
Subject(s) - raman scattering , raman spectroscopy , fluorescence , optics , materials science , microscopy , fluorescence lifetime imaging microscopy , optoelectronics , nuclear magnetic resonance , physics
Stimulated Raman scattering (SRS) has attracted increasing attention in bio-imaging because of the ability toward background-free molecular-specific acquisitions without fluorescence labeling. Nevertheless, the corresponding sensitivity and specificity remain far behind those of fluorescence techniques. Here, we demonstrate SRS spectro-microscopy driven by a multiple-plate continuum (MPC), whose octave-spanning bandwidth (600-1300 nm) and high spectral energy density (∼1 nJ/cm -1 ) enable spectroscopic interrogation across the entire Raman active region (0-4000 cm -1 ), SRS imaging of a Drosophila brain, and electronic pre-resonance (EPR) detection of a fluorescent dye. We envision that utilizing MPC light source will substantially enhance the sensitivity and specificity of SRS by implementing EPR mode and spectral multiplexing via accessing three or more coherent wavelengths.