Open AccessSplicing exponential point spread function design for localization of nanoparticles
Author(s) -
Hangfeng Li,
Famin Wang,
Xin Miao,
Wei Huang,
Yuanda Cheng,
Yun Xiao,
Tao Wei,
Yunhai Zhang
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.440721
Subject(s) - point spread function , point (geometry) , exponential function , point process , function (biology) , range (aeronautics) , optics , computer science , biological system , physics , materials science , mathematics , mathematical analysis , geometry , statistics , evolutionary biology , composite material , biology
We propose a point spread function for three-dimensional localization of nanoparticles. The axial detection range of the point spread function can be simply changed by adjusting the design parameters. In addition, the spatial extent of the point spread function can also be changed by adjusting the design parameters, which is an advantage other point spread functions do not have. We used our point spread functions and the existing point spread functions for dense multi-particle imaging, which proved the advantage that the point spread function with a smaller spatial extent we designed can effectively reduce the overlap between the point spread functions. The three-dimensional process of the fluorescent microsphere penetrating HT-22 cell membrane was successfully recorded, which verified the effectiveness of this method.