Open AccessIncoherent superposition of polychromatic light enables single-shot nondiffracting light-sheet microscopy
Author(s) -
Vahid Ebrahimi,
Jialei Tang,
Kyu Young Han
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.439338
Subject(s) - light sheet fluorescence microscopy , supercontinuum , optics , microscopy , superposition principle , materials science , super resolution microscopy , optical microscope , physics , scanning confocal electron microscopy , scanning electron microscope , wavelength , quantum mechanics , photonic crystal fiber
We demonstrate single-shot nondiffracting light-sheet microscopy by the incoherent superposition of dispersed polychromatic light sources. We characterized our technique by generating a Bessel light-sheet with a supercontinuum light-source and a C-light-sheet using a diode laser, and demonstrated its applicability to fluorescence microscopy. We emphasize that our method is easily implementable and compatible with the requirements of high-resolution microscopy.