Open AccessQuantification of HER2 in COS7 cells using quantum weak measurement
Author(s) -
Dongmei Li,
Chaofan Weng,
Kan Li,
Qiang Lin,
Jingjing Zhang,
Shuqian Wang,
Yi Ruan,
Jia Yao
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.437400
Subject(s) - trastuzumab , monoclonal antibody , interferometry , optics , materials science , chemistry , antibody , cancer , physics , breast cancer , biology , immunology , genetics
A Mach-Zehnder interferometer system based on weak measurement was set up to determinate the concentration variation of molecule by measuring the phase difference change between the two optical paths. The spectrum of the light was recorded to monitor the concentration of trastuzumab (Herceptin), which is a humanised monoclonal antibody, targeted to human epidermal growth factor receptor 2 (HER2). The trastuzumab targeting to HER2 was real-time detected and continuously monitored, the HER2 numbers of COS7 cells on a coverslip was determined at pico-molar level. Our weak measurement enabled method proposes an alternative approach for the concentration detection of molecules, providing a promising functional tool for the quantification of HER2 in cancer cells, possibly promoting fields such as the diagnosis and treatment of cancer.