Open AccessAdaptive optics with spatio-temporal lock-in detection for temporal focusing microscopy
Author(s) -
Tomohiro Ishikawa,
Keisuke Isobe,
Kenta Inazawa,
Kamiki,
Atsushi Miyawaki,
Fumihiko Kannari,
Katsumi Midorikawa
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.432414
Subject(s) - optics , wavefront , microscopy , adaptive optics , distortion (music) , pulse shaping , pulse duration , temporal resolution , ultrashort pulse , materials science , physics , laser , optoelectronics , amplifier , cmos
Wavefront distortion in temporal focusing microscopy (TFM) results in a distorted temporal profile of the excitation pulses owing to spatio-temporal coupling. Since the pulse duration is dramatically changed in the excitation volume, it is difficult to correct the temporal profile for a thick sample. Here, we demonstrate adaptive optics (AO) correction in a thick sample. We apply structured illumination microscopy (SIM) to an AO correction in wide-field TFM to decrease the change in the pulse duration in the signal detection volume. The AO correction with SIM was very successful in a thick sample for which AO correction with TFM failed.