Open AccessDistinguishing between coherent and incoherent signals in excitation-emission spectroscopy
Author(s) -
Daniel C. Lünemann,
Anitta R. Thomas,
Jingjing Xu,
Rabea Bartölke,
Henrik Mouritsen,
Antonietta De Sio,
Christoph Lienau
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.428850
Subject(s) - coherent spectroscopy , spectroscopy , optics , coherent anti stokes raman spectroscopy , excitation , fluorescence spectroscopy , raman scattering , microscopy , ultrashort pulse , materials science , time resolved spectroscopy , fourier transform spectroscopy , scattering , raman spectroscopy , physics , fluorescence , laser , fourier transform infrared spectroscopy , quantum mechanics
The separation of incoherent emission signals from coherent light scattering often poses a challenge in (time-resolved) microscopy or excitation-emission spectroscopy. While in spectro-microscopy with narrowband excitation this is commonly overcome using spectral filtering, it is less straightforward when using broadband Fourier-transform techniques that are now becoming commonplace in, e.g., single molecule or ultrafast nonlinear spectroscopy. Here we show that such a separation is readily achieved using highly stable common-path interferometers for both excitation and detection. The approach is demonstrated for suppression of scattering from flavin adenine dinucleotide (FAD) and weakly emissive cryptochrome 4 (Cry4) protein samples. We expect that the approach will be beneficial, e.g., for fluorescence lifetime or Raman-based imaging and spectroscopy of various samples, including single quantum emitters.