Open AccessImproved spatial resolution using focal modulation microscopy with a Tai Chi aperture
Author(s) -
Ao Deng,
Yian Zheng,
Jiajia Chen,
Yao Zheng,
Wei Gong,
Ke Si
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.426600
Subject(s) - optics , image resolution , microscopy , resolution (logic) , materials science , diffraction , confocal microscopy , microscope , image quality , spatial frequency , penetration depth , coded aperture , aperture (computer memory) , physics , detector , computer science , artificial intelligence , image (mathematics) , acoustics
Focal modulation microscopy (FMM) has gained significant interest in biological imaging. However, the spatial resolution and penetration depth limit the imaging quality of FMM due to the strong scattering background. Here, we introduce FMM with a Tai Chi aperture (TCFMM) based on diffraction theory to improve the spatial resolution. The results show that the transverse resolution is improved by 61.60% and 41.37% in two orthogonal directions, and the axial resolution is improved by 29.67%, compared with confocal microscopy (CM). The signal background ratio (SBR) of TCFMM is increased by 23.26% compared with CM and remains nearly the same compared with FMM using D-shape apertures (DFMM). These improvements in spatial resolution and SBR indicate that TCFMM has potential in deep tissue imaging.