Open AccessPupil-aberration calibration with controlled illumination for quantitative phase imaging
Author(s) -
YoonSeok Baek,
Hervé Hugonnet,
YongKeun Park
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.426080
Subject(s) - optics , phase imaging , pupil , calibration , materials science , phase (matter) , refractometry , resolution (logic) , sample (material) , refractive index , microscopy , computer science , physics , quantum mechanics , artificial intelligence , thermodynamics
Quantitative phase imaging (QPI) exploits sample-induced changes in the optical field to analyze biological specimens in a label-free manner. However, the quantitative nature of QPI makes it susceptible to optical aberrations. We propose a method for calibrating pupil aberrations by imaging a sample of interest. The proposed method recovers pupil information by utilizing the cross-spectral density between optical fields at different incident angles and allows both thin and weakly scattering three-dimensional samples for calibration. We experimentally validate the proposed method by imaging various samples, including a resolution target, breast tissue, and a polystyrene bead, and demonstrate aberration-free two- and three-dimensional QPI.