Open AccessAu-NPs signal amplification ultra-sensitivity optical microfiber interferometric biosensor
Author(s) -
Hanglin Lu,
Runjia Liu,
Peiyuan Liu,
Wenfu Lin,
Yan Huang,
Ruitao Xiao,
Zhenru Li,
Jun Ma,
Wei Wang,
Jie Li,
Li-Peng Sun,
BaiOu Guan
Publication year - 2021
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.424878
Subject(s) - microfiber , biosensor , detection limit , materials science , interferometry , oligonucleotide , dna , optics , dna–dna hybridization , nanotechnology , chemistry , physics , chromatography , biochemistry , composite material
An optical microfiber interferometric biosensor for the low concentration detection of sequence-specific deoxyribonucleic acid (DNA) based on signal amplification technology via oligonucleotides linked to gold nanoparticles (Au-NPs) is proposed and experimentally analyzed. The sensor uses a "sandwich" detection strategy, in which capture probe DNA (DNA-c) is immobilized on the surface of the optical microfiber interferometer, the reporter probe DNA (DNA-r) is immobilized on the surface of Au-NPs, and the DNA-c and DNA-r are hybridized to the target probe DNA (DNA-t) in a sandwich arrangement. The dynamic detection of the DNA-t was found to range from 1.0×10 -15 M to 1.0×10 -8 M, and the limit of detection (LOD) concentration was 1.32 fM. This sensor exhibited not only a low LOD but also excellent selectivity against mismatched DNA-t, and it can be further developed for application in various sensing platforms.