Open AccessFluorescence fluctuation-based super-resolution microscopy using multimodal waveguided illumination
Author(s) -
Ida S. Opstad,
Daniel Henry Hansen,
Sebastián Acuña,
Florian Ströhl,
Anish Priyadarshi,
Jean-Claude Tinguely,
Firehun Tsige Dullo,
Roy Ambli Dalmo,
Tore Seternes,
Balpreet Singh Ahluwalia,
Krishna Agarwal
Publication year - 2021
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.423809
Subject(s) - total internal reflection fluorescence microscope , microscopy , optics , superresolution , resolution (logic) , fluorescence microscope , fluorescence , total internal reflection , reflection (computer programming) , photonics , physics , image resolution , signal (programming language) , common emitter , temporal resolution , materials science , computer science , optoelectronics , computer vision , artificial intelligence , image (mathematics) , programming language
Photonic chip-based total internal reflection fluorescence microscopy (c-TIRFM) is an emerging technology enabling a large TIRF excitation area decoupled from the detection objective. Additionally, due to the inherent multimodal nature of wide waveguides, it is a convenient platform for introducing temporal fluctuations in the illumination pattern. The fluorescence fluctuation-based nanoscopy technique multiple signal classification algorithm (MUSICAL) does not assume stochastic independence of the emitter emission and can therefore exploit fluctuations arising from other sources, as such multimodal illumination patterns. In this work, we demonstrate and verify the utilization of fluctuations in the illumination for super-resolution imaging using MUSICAL on actin in salmon keratocytes. The resolution improvement was measured to be 2.2-3.6-fold compared to the corresponding conventional images.