Open AccessImprovement in image quality via the pseudo confocal effect in multidirectional digital scanned laser light-sheet microscopy
Author(s) -
Junichi Kaneshiro,
Go Shioi,
Kazuko Okamoto,
Shuichi Onami,
Tomonobu M. Watanabe
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.423783
Subject(s) - confocal , optics , light sheet fluorescence microscopy , confocal microscopy , image quality , materials science , confocal laser scanning microscopy , optical sectioning , microscopy , laser scanning , laser , microscope , digital imaging , image processing , scanning confocal electron microscopy , digital image , computer vision , biomedical engineering , computer science , image (mathematics) , physics , medicine
Multidirectional digital scanned laser light-sheet microscopy (mDSLM) cannot be used with the current pseudo confocal system to reduce blurring and background signals. The multiline scanning for light-sheet illumination and the simple image construction proposed in this study are alternative to the pseudo confocal system. We investigate the effectiveness of our pseudo confocal method combined with mDSLM on artificial phantoms and biological samples. The results indicate that image quality from mDSLM can be improved by the confocal effect; their combination is effective and can be applied to biological investigations.