Open AccessVirtual hematoxylin and eosin histopathology using simultaneous photoacoustic remote sensing and scattering microscopy
Author(s) -
Brendon S. Restall,
Nathaniel J. M. Haven,
Pradyumna Kedarisetti,
Matthew T. Martell,
Brendyn D. Cikaluk,
Sveta Silverman,
Lashan Peiris,
Jean Deschênes,
Roger J. Zemp
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.423740
Subject(s) - h&e stain , eosin , microscopy , confocal microscopy , optics , materials science , staining , confocal , eosin y , fluorescence microscope , fluorescence , pathology , biomedical engineering , chemistry , medicine , physics , biochemistry , photocatalysis , catalysis
Hematoxylin and eosin (H&E) staining is the gold standard for most histopathological diagnostics but requires lengthy processing times not suitable for point-of-care diagnosis. Here we demonstrate a 266-nm excitation ultraviolet photoacoustic remote sensing (UV-PARS) and 1310-nm microscopy system capable of virtual H&E 3D imaging of tissues. Virtual hematoxylin staining of nuclei is achieved with UV-PARS, while virtual eosin staining is achieved using the already implemented interrogation laser from UV-PARS for scattering contrast. We demonstrate the capabilities of this dual-contrast system for en-face planar and depth-resolved imaging of human tissue samples exhibiting high concordance with H&E staining procedures and confocal fluorescence microscopy. To our knowledge, this is the first microscopy approach capable of depth-resolved imaging of unstained thick tissues with virtual H&E contrast.