Open AccessExtended field of view of light-sheet fluorescence microscopy by scanning multiple focus-shifted Gaussian beam arrays
Author(s) -
Chao Liu,
Bai Chen,
Xianghua Yu,
Shaohui Yan,
Yuan Zhou,
Xing Li,
Junwei Min,
Yue Yang,
Dan Dan,
Baoli Yao
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.418707
Subject(s) - light sheet fluorescence microscopy , optics , optical sectioning , gaussian beam , focus (optics) , perpendicular , gaussian , resolution (logic) , photobleaching , image resolution , microscopy , materials science , field of view , physics , beam (structure) , fluorescence , scanning confocal electron microscopy , computer science , artificial intelligence , geometry , mathematics , quantum mechanics
Light-sheet fluorescence microscopy (LSFM) facilitates high temporal-spatial resolution, low photobleaching and phototoxicity for long-term volumetric imaging. However, when a high axial resolution or optical sectioning capability is required, the field of view (FOV) is limited. Here, we propose to generate a large FOV of light-sheet by scanning multiple focus-shifted Gaussian beam arrays (MGBA) while keeping the high axial resolution. The positions of the beam waists of the multiple Gaussian beam arrays are shifted in both axial and lateral directions in an optimized arranged pattern, and then scanned along the direction perpendicular to the propagation axis to form an extended FOV of light-sheet. Complementary beam subtraction method is also adopted to further improve axial resolution. Compared with the single Gaussian light-sheet method, the proposed method extends the FOV from 12 μm to 200 μm while sustaining the axial resolution of 0.73 μm. Both numerical simulation and experiment on samples are performed to verify the effectiveness of the method.