Comparative study on resolution enhancements in fluorescence-structured illumination Fresnel incoherent correlation holography | Zendy

Philjun Jeon | Zendy; Jongwu Kim | Zendy; Hee-Jung Lee | Zendy; HyukSang Kwon | Zendy; Dug Young Kim | Zendy

Open Access

Comparative study on resolution enhancements in fluorescence-structured illumination Fresnel incoherent correlation holography

Author(s) -

Philjun Jeon,

Jongwu Kim,

Hee-Jung Lee,

HyukSang Kwon,

Dug Young Kim

Publication year - 2021

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.417206

Subject(s) - holography , optics , microscopy , resolution (logic) , scattering , fluorescence correlation spectroscopy , fluorescence microscope , image resolution , physics , light scattering , fluorescence , materials science , computer science , artificial intelligence

Fresnel incoherent correlation holography (FINCH) is a new approach for incoherent holography, which also has enhancement in the transverse resolution. Structured illumination microscopy (SIM) is another promising super-resolution technique. SI-FINCH, the combination of SIM and FINCH, has been demonstrated lately for scattering objects. In this study, we extended the application of SI-FINCH toward fluorescent microscopy. We have built a versatile multimodal microscopy system that can obtain images of four different imaging schemes: conventional fluorescence microscopy, FINCH, SIM, and SI-FINCH. Resolution enhancements were demonstrated by comparing the point spread functions (PSFs) of the four different imaging systems by using fluorescence beads of 1-μm diameter.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research