Open AccessComparative study on resolution enhancements in fluorescence-structured illumination Fresnel incoherent correlation holography
Author(s) -
Philjun Jeon,
Jongwu Kim,
Hee-Jung Lee,
Hyuk Sang Kwon,
Dug Young Kim
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.417206
Subject(s) - holography , optics , microscopy , resolution (logic) , scattering , fluorescence correlation spectroscopy , fluorescence microscope , image resolution , physics , light scattering , fluorescence , materials science , computer science , artificial intelligence
Fresnel incoherent correlation holography (FINCH) is a new approach for incoherent holography, which also has enhancement in the transverse resolution. Structured illumination microscopy (SIM) is another promising super-resolution technique. SI-FINCH, the combination of SIM and FINCH, has been demonstrated lately for scattering objects. In this study, we extended the application of SI-FINCH toward fluorescent microscopy. We have built a versatile multimodal microscopy system that can obtain images of four different imaging schemes: conventional fluorescence microscopy, FINCH, SIM, and SI-FINCH. Resolution enhancements were demonstrated by comparing the point spread functions (PSFs) of the four different imaging systems by using fluorescence beads of 1-μm diameter.