Open AccessHadamard-transform spectral acquisition with an acousto-optic tunable filter in a broadband stimulated Raman scattering microscope
Author(s) -
Luca Genchi,
Andrea Bucci,
Sergey P. Laptenok,
Alessandro Giammona,
Carlo Liberale
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.415752
Subject(s) - optics , materials science , raman scattering , hadamard transform , multiplexing , raman spectroscopy , laser , narrowband , physics , optoelectronics , computer science , telecommunications , quantum mechanics
We present a novel configuration for high spectral resolution multiplexing acquisition based on the Hadamard transform in stimulated Raman scattering (SRS) microscopy. The broadband tunable output of a dual-beam femtosecond laser is filtered by a fast, narrowband, and multi-channel acousto-optic tunable filter (AOTF). By turning on and off different subsets of its 8 independent channels, the AOTF generates the spectral masks given by the Hadamard matrix. We demonstrate a seamless and automated operation in the Raman fingerprint and CH-stretch regions. In the presence of additive noise, the spectral measurements using the multiplexed method show the same signal-to-noise ratio of conventional single-wavenumber acquisitions performed with 4 times longer integration time.