Open AccessAxially resolved volumetric two-photon microscopy with an extended field of view using depth localization under mirrored Airy beams
Author(s) -
Ryan K Y Chan,
Hongsen He,
Yu-Xuan Ren,
Cora Sau Wan Lai,
Edmund Y. Lam,
Kenneth K. Y. Wong
Publication year - 2020
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.412453
Subject(s) - optics , image resolution , axial symmetry , microscopy , resolution (logic) , iterative reconstruction , depth of field , field of view , sample (material) , temporal resolution , physics , field (mathematics) , computer science , computer vision , artificial intelligence , mathematics , quantum mechanics , thermodynamics , pure mathematics
It is a great challenge in two-photon microscopy (2PM) to have a high volumetric imaging speed without sacrificing the spatial and temporal resolution in three dimensions (3D). The structure in 2PM images could be reconstructed with better spatial and temporal resolution by the proper choice of the data processing algorithm. Here, we propose a method to reconstruct 3D volume from 2D projections imaged by mirrored Airy beams. We verified that our approach can achieve high accuracy in 3D localization over a large axial range and is applicable to continuous and dense sample. The effective field of view after reconstruction is expanded. It is a promising technique for rapid volumetric 2PM with axial localization at high resolution.