Open AccessMeasuring the spatial distribution of multiply scattered light using a de-scanned image sensor for examining retinal structure contrast
Author(s) -
Benjamin S. Sajdak,
Jack Postlewaite,
Kevin W. Eliceiri,
Jeremy D. Rogers
Publication year - 2021
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.408587
Subject(s) - optics , contrast (vision) , retinal , pixel , spatial frequency , image sensor , microscope , image contrast , light scattering , microscopy , image processing , image resolution , computer science , physics , materials science , artificial intelligence , image (mathematics) , scattering , biochemistry , chemistry
An optical platform is presented for examining intrinsic contrast detection strategies when imaging retinal structure using ex vivo tissue. A custom microscope was developed that scans intact tissue and collects scattered light distribution at every image pixel, allowing digital masks to be applied after image collection. With this novel approach at measuring the spatial distribution of multiply scattered light, known and novel methods of detecting intrinsic cellular contrast can be explored, compared, and optimized for retinal structures of interest.