Open AccessVolumetric chemical imaging in vivo by a remote-focusing stimulated Raman scattering microscope
Author(s) -
Peng Lin,
Hongli Ni,
Huate Li,
Nicholas A. Vickers,
Yuying Tan,
Ruyi Gong,
Thomas G. Bifano,
JiXin Cheng
Publication year - 2020
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.404869
Subject(s) - microscope , optics , materials science , chemical imaging , microscopy , raman scattering , biological imaging , lens (geology) , optical microscope , preclinical imaging , raman spectroscopy , scanning electron microscope , in vivo , remote sensing , physics , microbiology and biotechnology , hyperspectral imaging , fluorescence , biology , geology
Operable under ambient light and providing chemical selectivity, stimulated Raman scattering (SRS) microscopy opens a new window for imaging molecular events on a human subject, such as filtration of topical drugs through the skin. A typical approach for volumetric SRS imaging is through piezo scanning of an objective lens, which often disturbs the sample and offers a low axial scan rate. To address these challenges, we have developed a deformable mirror-based remote-focusing SRS microscope, which not only enables high-quality volumetric chemical imaging without mechanical scanning of the objective but also corrects the system aberrations simultaneously. Using the remote-focusing SRS microscope, we performed volumetric chemical imaging of living cells and captured in real time the dynamic diffusion of topical chemicals into human sweat pores.