Open Access7X multiplexed, optofluidic detection of nucleic acids for antibiotic-resistance bacterial screening
Author(s) -
Gopikrishnan G. Meena,
Thomas A. Wall,
Matthew A. Stott,
O. Brown,
Richard A. Robison,
Aaron R. Hawkins,
Holger Schmidt
Publication year - 2020
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.402311
Subject(s) - nucleic acid , multiplexing , antibiotic resistance , interference (communication) , antibiotics , microbiology and biotechnology , computer science , biology , genetics , telecommunications , channel (broadcasting)
Rapid and accurate diagnosis of bacterial infections resistant to multiple antibiotics requires development of new bio-sensors for differentiated detection of multiple targets. This work demonstrates 7x multiplexed detection for antibiotic-resistance bacterial screening on an optofluidic platform. We utilize spectrally multiplexed multi-spot excitation for simultaneous detection of nucleic acid strands corresponding to bacterial targets and resistance genes. This is enabled by multi-mode interference (MMI) waveguides integrated in an optofluidic device. We employ a combinatorial three-color labeling scheme for the nucleic acid assays to scale up their multiplexing capability to seven different nucleic acids, representing three species and four resistance genes.