Method for monitoring singlet oxygen quantum yield in real time by time resolved spectroscopy measurement | Zendy

Ting Liu | Zendy; Xiyu Zhang | Zendy; Honglin Zhang | Zendy; Hua Zhao | Zendy; Zhiguo Zhang | Zendy; Ye Tian | Zendy

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Method for monitoring singlet oxygen quantum yield in real time by time resolved spectroscopy measurement

Author(s) -

Ting Liu,

Xiyu Zhang,

Honglin Zhang,

Hua Zhao,

Zhiguo Zhang,

Ye Tian

Publication year - 2020

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.401423

Subject(s) - optics , spectroscopy , singlet oxygen , time resolved spectroscopy , quantum yield , materials science , yield (engineering) , physics , oxygen , optoelectronics , quantum mechanics , metallurgy , fluorescence

The singlet oxygen quantum yield (Φ Δ ) was monitored in real time through time resolved spectroscopy measurement, using gadolinium labeled hematoporphyrin monomethyl ether (Gd-HMME) as photosensitizer. According to the kinetics equations of singlet oxygen generation and reaction, Φ Δ was related to phosphorescence lifetime (τ p ). Through measuring τ p of Gd-HMME in different oxygen conditions, the radiation transition property of first exited triplet state (T 1 ) was monitored; combined with the triplet state quantum yield (Φ T ) determined by linear fitting the Φ Δ , which was measured in different oxygen content using a relative measurement, Φ Δ can be determined in real time. The identification of anoxia during the treatment of photodynamic therapy (PDT) by this method is also presented.

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