Open AccessMethod for monitoring singlet oxygen quantum yield in real time by time resolved spectroscopy measurement
Author(s) -
Ting Liu,
Xiyu Zhang,
Honglin Zhang,
Hua Zhao,
Zhiguo Zhang,
Ye Tian
Publication year - 2020
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.401423
Subject(s) - singlet oxygen , photosensitizer , phosphorescence , quantum yield , spectroscopy , hematoporphyrin , oxygen , triplet state , materials science , photochemistry , limiting oxygen concentration , time resolved spectroscopy , yield (engineering) , analytical chemistry (journal) , chemistry , photodynamic therapy , optics , atomic physics , fluorescence , excited state , physics , chromatography , metallurgy , organic chemistry , quantum mechanics
The singlet oxygen quantum yield (Φ Δ ) was monitored in real time through time resolved spectroscopy measurement, using gadolinium labeled hematoporphyrin monomethyl ether (Gd-HMME) as photosensitizer. According to the kinetics equations of singlet oxygen generation and reaction, Φ Δ was related to phosphorescence lifetime (τ p ). Through measuring τ p of Gd-HMME in different oxygen conditions, the radiation transition property of first exited triplet state (T 1 ) was monitored; combined with the triplet state quantum yield (Φ T ) determined by linear fitting the Φ Δ , which was measured in different oxygen content using a relative measurement, Φ Δ can be determined in real time. The identification of anoxia during the treatment of photodynamic therapy (PDT) by this method is also presented.